Language
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Membrane |
Polyethersulfone |
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Gaskets |
Pvdf ort Thermoplastic elastomer |
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Spacer |
Polipropylene |
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Preservative |
1.6% Phosphoric Acid, |
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Sealing compound |
Silicone white |
Benefits of Production TFF System
Scalability
TFF handles volumes from a few millilitres to many litres using the same fundamental setup, simply by selecting an appropriately sized filter module. This is a significant advantage over ultracentrifugation, which is constrained by rotor capacity.
Gentle Processing
The tangential flow mechanism minimises the mechanical stress on EVs. Unlike the extreme g-forces of ultracentrifugation (≥100,000×g), TFF operates at modest pressures and flow rates, helping to preserve EV integrity and biological activity.
Reproducibility
Because TFF parameters such as TMP, cross-flow rate and diafiltration volumes can be precisely controlled, the process is inherently more reproducible than many manual isolation methods.
Concentration And Diafiltration In One System
TFF can both concentrate EVs and exchange the buffer in a single workflow, reducing sample handling steps and the associated risk of losses.
Removal Of Soluble Contaminants
Free proteins, lipoproteins, and media supplements smaller than the membrane MWCO are efficiently washed through as permeate, improving the purity of the EV preparation.
Transmembrane Pressure (TMP) is the driving force that pushes solvent and small molecules through the membrane. It is calculated as the average of the feed and retentate pressures minus the permeate pressure. TMP needs to be carefully controlled: too low and throughput suffers; too high and a dense “gel layer” of retained material forms on the membrane surface, paradoxically reducing flux and potentially trapping EVs.
Cross-Flow Rate (CFR) is the velocity of the feed stream as it moves across the membrane surface. A higher cross-flow creates more sweeping force, keeping the membrane surface cleaner and maintaining flux. However, excessively high cross-flow rates generate shear stress that can damage sensitive biological particles such as EVs. Finding the right balance between sufficient sweeping action and acceptable shear is one of the key optimisation steps in TFF.
Permeate Flux describes the rate at which liquid passes through the membrane, typically expressed as litres per square metre per hour (LMH). A well-optimised system operates at a stable flux that represents the best compromise between processing speed and membrane longevity. Monitoring flux over time also serves as a real-time indicator of membrane fouling.
Molecular Weight Cut-Off (MWCO) defines the nominal size threshold of the membrane. Molecules below the MWCO pass through as permeate, while those above are retained.
In conventional “dead-end” filtration, a sample is pushed directly through a membrane. Everything larger than the membrane’s pore size accumulates on the surface, forming a progressively thicker cake layer that reduces flow and can damage delicate particles. Anyone who has tried to concentrate a litre of conditioned medium through a centrifugal filter will recognise the diminishing returns.
TFF takes a fundamentally different approach. Instead of forcing the sample perpendicularly through the membrane, the feed stream flows tangentially, in other words parallel to the membrane surface. This sweeping action continuously lifts retained material away from the membrane, dramatically reducing fouling and allowing the system to process large volumes without the progressive loss of flux that plagues dead-end methods.
Flexible Global Shipping Solutions
We support multiple shipping methods including sea freight, air freight, rail transportation, and express delivery to meet different delivery timelines and project requirements. Professional packaging and export handling ensure safe and efficient transportation for international orders.
As one of the leading production tff system manufacturers and suppliers in China, we also support customized service. We warmly welcome you to wholesale high quality production tff system in stock here from our factory. For quotation, contact us now.
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