Laboratory Protein Purification System

 

The Jiuling Laboratory TFF System is engineered specifically for protein concentration, diafiltration, and buffer exchange in early-stage bioprocess development.
Designed to bridge the gap between bench-scale R&D and commercial manufacturing, this system allows researchers to evaluate filtration performance, optimize critical process parameters (CPP), and establish stable operating windows. By delivering exceptional linear scale-up consistency, it minimizes process re-optimization risks when transitioning to pilot and production scales.

 

 

Item	Specification
System Type	Lab-scale TFF system
Operation Mode	Concentration / Diafiltration / Buffer Exchange
Flow Rate	10 mL/min – 2 L/min
Pressure Range	0 – 4 bar
Membrane Types	Hollow fiber / cassette
MWCO Range	1 kDa – 750 kDa+
Pump Type	Peristaltic pump
Materials	316L SS, PTFE, PVDF, silicone
Control Mode	Manual / semi-digital

 

 

Stable TMP Control: Fluctuating Transmembrane Pressure (TMP) is a primary driver of protein aggregation, severe membrane fouling, and low yield. This system utilizes real-time pressure monitoring to maintain a highly stable, tightly controlled TMP profile during both concentration and diafiltration phases.
Ultra-Low Hold-Up Volume: The fluidics flow path is optimized to minimize internal dead volume. This engineering focus drastically reduces sample retention and product loss, making it highly efficient for low-concentration yields or high-value biologics.
Scale-Up Consistency: The system's hydraulic design, channel configurations, and flow dynamics closely mimic larger pilot-scale TFF machinery. This alignment minimizes re-optimization bottlenecks and risk when scaling up processes from the bench to commercial manufacturing.
Controlled Shear Conditions: By pairing the system with precise pump kinetics and a smooth flow-path layout, fluid shear stress is strictly managed, preserving the biological activity and confirmation of shear-sensitive target molecules.

 

 

Antibody Purification: Downstream concentration and formulation of mAbs and recombinant antibodies.
Recombinant Proteins: Biomass volume reduction and concentration of engineered proteins post-chromatography.
Enzyme Recovery: Gentle isolation and concentration of industrial and medical enzymes.
Vaccines & Viral Vectors: Processing and purification of live/attenuated vaccines, Virus-Like Particles (VLPs), and gene therapy vectors.
Bioprocess Optimization: Establishing Critical Process Parameters (CPP) during early-stage downstream development.

 

Pump System: High-precision peristaltic pump featuring a broad, adjustable flow rate.
Pressure Management: Real-time digital monitoring for both Feed and Retentate channels (Optional automated TMP calculation module available).
Filter Compatibility: Universal open-architecture holder design; fully compatible with both Hollow Fiber cartridges and Flat-Sheet cassettes across major global brands.
Membrane Cutoff Accommodations: Supports a wide MWCO range from 1 kDa to over 750 kDa.
Tubing Circuit: Low-adsorption, ultra-low hold-up volume pharmaceutical-grade tubing assembly with specialized quick-connect fittings for rapid, tool-free installation.

 

 

While standard laboratory TFF devices on the market provide basic filtration capabilities, the Jiuling system delivers distinctive, engineering-led advantages for bioprocess scaling:
Minimized Sample Loss: Tailored flow paths reduce dead volume significantly compared to generic laboratory loops, boosting yields on scarce R&D batches.
Low-Flow Stability: Maintains rock-solid TMP control even when running at the lower limits of its flow rate spectrum.
Vendor-Agnostic Flexibility: Avoids vendor lock-in; open-source hardware allows the running of your preferred choice of global membrane brands.
Seamless Tech Transfer: Built with the specific geometric and fluidic properties required to predictably scale data upward to pilot plants.

 

 

Membrane Modules: Specialized adapters for distinct hollow fiber or cassette geometries.
Fluidics Optimization: Custom tailored flow-rate ranges and specialized low-volume tubing manifolds.
Process Vessels: Variable volume jacketed or non-jacketed feed reservoirs with custom port layouts.
Sensor Integration: Upstream/downstream pressure transducers, UV, pH, or conductivity sensor configurations.
Automation Upgrades: Scale expansions from basic manual operation to fully automated, data-logging control systems.
OEM/ODM Services: Complete contract engineering and white-label manufacturing capabilities for global system integrators.

 

 

To guarantee strict compliance with laboratory QA and validation protocols, every Jiuling TFF system undergoes comprehensive Factory Acceptance Testing (FAT) before global shipment:
Pressure Integrity Test: High-pressure leak testing across the entire fluid loop up to maximum rated operating limits (4 bar).
Flow Stability Calibration: Dynamic verification of pump performance and flow consistency over extended run times.
Sensor Verification: Multi-point calibration check against traceable standards for all pressure sensors.
System Integrity Audit: Full visual and functional inspection of all physical assemblies, wetted components, and mechanical connections.

 

 

Industrial Shock Isolation: Heavy-duty, multi-layer export crating designed to survive international freight handling.
Isolated Component Segregation: Critical components, sensors, and delicate electronics are individually packed in anti-static, shock-absorbing cells.
Environmental Barrier: Sealed moisture-proof, vapor-barrier inner packaging to protect components from maritime and transit humidity.
Documentation Suite: Shipped with a comprehensive, step-by-step installation manual, quick-start guide, and material conformity certificates.

 

 

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