Protein Concentration Tangential Flow Filtration System

 

Jiuling designs and supplies Tangential Flow Filtration (TFF) systems for downstream bioprocess applications including protein concentration, buffer exchange, and purification preparation.
Each system is configured based on process conditions such as flow rate, membrane selection, and automation level, rather than fixed equipment models. This approach helps maintain consistency during scale-up and routine production.

 

 

The system operates on tangential flow filtration, where the feed stream flows parallel to the membrane surface under controlled crossflow conditions.
This reduces direct particle accumulation and helps maintain stable filtration performance over long operating cycles.
Compared with dead-end filtration, this method is more suitable for protein solutions that require structural stability and high recovery rates.

 

 

Parameter	Specification
Filtration Mode	Tangential Flow (Crossflow)
Application	Protein concentration / diafiltration / purification prep
Membrane Types	Hollow fiber / flat sheet / spiral wound
MWCO Range	1 kDa – 1000 kDa (customizable)
Filtration Area	0.1 m² – 20 m²+
Operating Pressure	Up to 6 bar
Temperature Range	4–40°C
Flow Mode	Recirculation / single-pass optional
Pump Type	Peristaltic / diaphragm / centrifugal
Control System	PLC-based (Siemens or equivalent)
Monitoring	Pressure / flow / TMP / temperature
Materials	316L stainless steel + biocompatible tubing
Cleaning	CIP / SIP optional

 

Reduced Scale-Up Risk
Lab, pilot, and production systems follow the same filtration principle. Scale-up is achieved through membrane area expansion rather than process redesign, reducing validation effort and transfer uncertainty.
Stable Process Performance
Crossflow operation helps maintain consistent flux behavior during long runs, reducing sudden performance drop caused by membrane fouling.
Protein Stability Protection
Operating conditions are designed to reduce mechanical stress, supporting stable recovery of sensitive proteins such as monoclonal antibodies and recombinant proteins.
Process Integration Compatibility
The system can be integrated into existing upstream and downstream lines without changing overall process architecture.

 

 

Protein Concentration
Concentrates target biomolecules by removing water and low molecular weight impurities while maintaining protein stability under controlled shear conditions.
Diafiltration (Buffer Exchange)
Supports continuous buffer replacement without precipitation or centrifugation, improving control over formulation conditions.
Pre-Chromatography Preparation
Reduces feed volume and improves homogeneity before chromatography, supporting more stable column loading performance.

 

 

The system is typically used in the downstream process chain:

• Cell culture harvest
• Clarification
• TFF concentration and buffer exchange
• Intermediate purification preparation
• Chromatography feed conditioning

By standardizing feed quality before chromatography, it helps improve downstream process consistency and reduce variability between batches.

 

 

 

 

System Preparation & Flush: Automated flushing and sanitization of the membrane loop.
Feed Transfer: Controlled transfer of the clarified harvest from the bioreactor or buffer tank.
Concentration Phase: Crossflow circulation with real-time, automated TMP adjustments to safely drive volume reduction.
Diafiltration Phase (Optional): Automated buffer addition matches permeate removal rate for precise salt or buffer exchange.
Product Recovery: Low-holdup volume recovery of the final concentrated intermediate into formulation or storage vessels.

 

Every Jiuling system is engineered and packed to withstand rigorous international transit conditions:
Skid-Mounted Frame: Compact, rigid stainless-steel frame, securely anchored for long-distance maritime or air freight.
Component Protection: High-grade shock isolation for sensitive instruments, PLC enclosures, and pumps.
Environmental Sealing: Moisture-resistant, anti-corrosion barrier packaging for all tubing, valves, and membrane connection ports.
Pre-Shipment Verification: Comprehensive Factory Acceptance Testing (FAT) conducted prior to dispatch. Systems can be delivered as a fully assembled single skid or modular assemblies for restricted-space on-site installation.

 

 

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